This is the first episode to feature Grass-type Pokémon's immunity to Leech Seed, as Clemont's Chespin was seen being unaffected by Jessie's Pumpkaboo's Leech Seed's energy absorption.
Team Rocket doesn't recite their motto in this episode.
Jessie catches a Pumpkaboo.
At first, Meowth blasts off at different time than Jessie and James, but at the end of the episode, the trio blasts off together.
Clemont's Chespin is revealed to know Vine Whip.