Guest characters appearing in the squares of the "Tic Tac Toony" grid are Tessa the production assistant, celebrity trumpeter Chuck Mangione, Todd Herling ("Horror-Anthology Host", spoofing
Rod Serling on
The Twilight Zone (1959)), radio shock jock Simon Shock, clumsy fitness guru Flex Armswrong ("Armed & Dangerous"), street mime Imena Bocks, Bill's Mom & Dad, backwoods funnyman Benny the IT Feller, and pop-culture aficionado Felton Fanboy. Todd Herling makes his first appearance since his debut in
The Toony Zone (2022), and Chuck Mangione (portrayed by
Bill Leff) is seen for the first time on the show.