Diane Cary (Beverly Barnes) in real life is married to James D. Parriott, creator of "Defying Gravity".
This episode's title is derived from "to cross the Rubicon". When Julius Caeser crossed the Rubicon River in 49 BC, he knew that doing so was making an unirrevocable choice that would change the future.
The container from which Jen Crane takes the rabbit embryo to show it is actually a Dymo label printer.