It is unknown what happened to the Rowdyruff Boys, as they never appear again after this.
Mojo breaks the fourth wall in this episode when he says "Nothing, like the amount of respect I get after 6 seasons on this show."
This episode was planned to be a 22-minute episode in order to explain what happened to the Rowdyruff Boys after this episode.
Strangely, The Rowdyruff Boys do not remember Mojo Jojo even though he was the one who created them, and even though they remember The Powerpuff Girls. It has been suggested that HIM altered their memories.
This is the first and only episode where HIM is seen crying.