All three Clones have visible scars on the sides of their heads where they had their control chips surgically removed. The existence of inhibitor chips in the clones was established in season six of The Clone Wars when the clone Fives discovered them, removed his own chip, and died trying to get the word out. Rex was the only clone to know about the chips. This episode aired before the final season of The Clone Wars which depicts how Rex's chip is removed. Inhibitor chips are further explored in The Bad Batch (2021-)
This marks Gregor's second appearance since the Clone Wars episode Missing in Action (2013) where he apparently sacrificed his life to help the D-Squad escape the planet Abafar. He is shown to have gone insane.
Sabine has a new blue hair color and repainted armor. Her right shoulder armor, which used to feature a checkered Dejarik pattern, now sports the number 5, for 'Spectre 5'. Her left shoulder armor, which used to have a drawing of an Anooba on it, now has a Fyrnock.
Agent Kallus has had a promotion, as made evident by his different rank badge and collar.
The Joopa fishing harness that Zeb wears is made from a Clone Wars-era backpack, used by Ahsoka to carry Rotta the Hutt in Star Wars: The Clone Wars (2008)