The film was originally announced for a 2000 release, but Full Moon's financial hardships delayed the production for decades. The studio eventually rebounded, and it was slated to be shot in 2020, but worldwide COVID lockdowns resulted in another production delay.
Filming was also originally set to take place in Albania before being re-located to Serbia. Croatia had also been considered for a shooting location.
When the film was announced for a 2000 release, filming at the time was to commence in Romania, akin to the previous Subspecies films, including its spin-off, Vampire Journals.
In this film, Denise Duff plays as the vampire that turns Anders Hove into one instead of it being like the other films where she's being chased and hounded by him.
The first entry in the Subspecies franchise to not be filmed in Romania.