In their final attempt to escape, Jake and Angela go out of their way to find a key to a door, even though the firemen were explicitly stated to be equipped with "Tools; really anything we might need to break a lock or open a door."
Although it's a necessary resource for the finale, the main character's video camera model does not have a "night-vision" feature. This function is commonly found on domestic Handycam camcorders but hardly on a big professional news reporter camera.
(at around 25 mins) Although dramatic, when the film crew, firefighter, and LAPD officer encounter an infected "Ms. Espinoza" and she is ultimately shot by the officer, neither would simply leave her (after being shot) without so much as checking her vitals. This is especially true as this was in the beginning when they are still trying to figure out what is going on.
The LAPD badges are not accurate.
(at around 1h 8 mins) Power to the building has been cut, yet the elevator still works. One character briefly mentioned that the building has a back-up generator, most likely to assist evacuation during emergencies. A pre-programmed back-up system explains why the elevator stops at each floor, and why the doors take so long to close.
(at around 51 mins) When the bio-suited CDC doctors enter the building, you hear the sounds of a self-contained breathing apparatus. However, the doctors are wearing standard gas masks with NBC filters, which are almost silent and don't use an external air supply. However, on the DVD/Blu-ray commentary for this film, the director/executive producer explain that because there was no musical score for this picture, they chose to "score" it using sound effects throughout. This is evidenced on the filmmaker commentary in the above scene, where the filmmakers further discuss the process by which the post-production sound department began creating sound effects as soon as the film editing process began, thus allowing the editor to "test out different breathing sounds" to see what worked best for dramatic effect. One could deduce that the sound effects used were intentionally inaccurate so as to add to the suspense of the moment rather than to be factually accurate.
(at around 1h 15 mins) Near the end, an article on the lab's wall has the headline "Doomsday Cult Suspected in Weapons Lab Break In," but the text is unrelated to the title. It is a copy of "Dead by 34: How AIDS and Starvation Condemn Zimbabwe's Women to An Early Grave" by Daniel Howden, November 17, 2006.
When the firefighters leave in the firetruck, with the News Crew, to respond to the emergency, you can hear the engine noise change on the firetruck each time the driver changes gears. The firetrucks depicted in this 2008 movie did not have a transmission that required the gears to be changed (standard transmission). The transmission is an automatic. The gears change automatically, and make very little noise when they change.
(at around 48 mins) Lawrence says the only way to test for rabies is a brain sample; a blood test will not work. A brain sample is the most reliable way to diagnose rabies, but it can also be diagnosed through saliva, urine, or cerebro-spinal fluid samples.
It makes absolutely no sense that Angela would continually demand that the camera man shine his light on her face so that she can see better while they are terrified and walking through the dark upstairs rooms. Doing this would only illuminate her face and blind her even more.
After the cop gets bitten by miss Espinoza, Jake asks for a doctor, and Lawrence, a vet, steps in to assist. However, as a fireman, Jake has had medical training, and at the start of the movie, the chief notes that most firemen are also paramedics, and that most of their calls are medical emergencies. As a result, Jake should be much more qualified to help here than a vet.