The original 1966 comic book story of the same name ("La Schtroumpfette" in French) differs greatly from the cartoon version:
- Gargamel created Smurfette so that she would use her charms to cause jealousy and competition among the Smurfs in order to cause their fall. She was not an actual Gargamel spy and saboteur who intentionally tries to disrupt life in the village as depicted in the cartoon version.
- She was transformed to her beautiful appearance by Papa Smurf via three days of magic performed on her instead of unspecified hours of magic like the cartoon version. The reason for the transformation was that Papa Smurf took pity of Smurfette for the abuse she received from the other Smurfs who saw her as unattractive and annoying, unlike the cartoon version where Smurfette wanted to become a real Smurf and break her ties to Gargamel.
- The water dam incident involving Smurfette took place after her transformation, not before it like the cartoon version. Unlike the cartoon version, she enticed Poet Smurf, not Greedy Smurf (who is a combination of two Smurfs: Greedy and Cook/Baker in the cartoons) to open the water dam.
- During the trial scene, Smurfette was seen by the other Smurfs as innocent (due to her passion for her) instead of guilty as found in the cartoon version.
- Gargamel did manipulate Smurfette to lure the Smurfs into a trap as depicted in the cartoon version, while in the original comics, he never left his home and is ignorant of her activities.
- Smurfette at the end of the original story chose to leave the village instead of staying as she did in the cartoon version. The reason she did so is to stop any more fighting among the Smurfs due to their love for her. In the early gags and latter stories, Smurfette will occasionally return to the Smurf Village, but she will eventually stay in it permanently without explanation by the 1980s.