While this film features the same character designs, general look, style, and most same cast members of as Superman: The Animated Series (1996), the creative team decided to recast villains just as they had done before with Batman: Mystery of the Batwoman (2003). Lance Henriksen takes over for Corey Burton as Brainiac, Powers Boothe takes over for Clancy Brown as Lex Luthor, and 'Tara Strong' takes over for Lisa Edelstein as Mercy Graves.
While Brainiac is downloading the LexLabs weapon files, at 6:01 among them are the five-legged spider robot introduced in the Jonny Quest cartoon The Robot Spy (1964) as well as the Quest Dragonfly jet plane.