Matthew is wearing leather riding gloves inside the cabin as the siblings gather supplies. When the soldiers interrupt the task and the siblings answer the door, Matthew is not wearing the gloves. He then runs to see Sully and Dr. Quinn, he is definitely bare handed.
Matthew's hat was black, and now it is grey.
Near the 25 min mark, Reverend Johnson's beard appears fake and the wrong color.
Near the 17 min mark, there is a horsefly on the back of Dorothy's dress near her right shoulder.
The baby doll Colleen is holding near the 35 min mark is only half the size of Katie.
Dorothy is wearing a dress with a zipper in the back during her discussion with Dr. Quinn. They are standing in the street talking about the current situation.
Dorothy is riding into the forest in a dress, hat, and gloves. She would have / should have also had a coat or cloak.
Once Dr. Quinn remove all Sully's dead skin, she needed to seal the wound with a hot iron. Ahe could not use chloroform during the first step. She definitely could had useful chloroform during the second step. Sully did not deserve to be alert during his branding.
Matthew is helping his two siblings gather supplies in the homestead. Matthew is in the cabin. He is wearing his leather rising gloves. This does not make sense.
Loren is going to Sully's funeral. Most of the other men are wearing hats. Loren is not wearing a hat. As a leading citizen and the owner of the town store, he should have been wearing a hat.