The birthmark on Alex's paw appears and disappears between shots.
When the penguins steal the tour jeep, the tour guide has no gun yet he has one when the humans pick up the old lady after the penguins leave with the jeep.
There is a shot in which the monkey is cutting through the middle frame with a grinder, then in the next shot he actually completes the task on the rear one.
When they ram the dam you can clearly see the old lady purse fly into the water and go down with the dam but they later give it to Makunga.
After Nana beats up Alex on the road, she gets back on the tour jeep and it drives away. Alex picks up her purse, dumps it out and drops it on the ground. Later, after the penguins hijack the jeep and drive over Nana, her fellow tourist says "Lady, I found your pocketbook," and returns it to her, when they were obviously a lot farther down the road from the initial Alex and Nana fight location.
The stripes on a zebra are much like the fingerprints on a human being, so none of the zebras in Marty's herd should look alike.
Lions do not have opposable thumbs. All the lions shown in this series have them.
When the little old lady and Alex fight, he's punched in the mouth and spits out a tooth. Said tooth looks exactly like a human molar, but lions' back teeth are shearing carnassials, not grinding molars.
The title, "Madagascar: Escape 2 Africa," might appear to be misleading since Madagascar can be considered part Africa. However, whether Madagascar is strictly part of Africa is perhaps geographically debatable, and certainly politically debatable. Technically, Madagascar is an island east of Africa, separate from the mainland. Politically, there is no pan-African government, and attempts to have Madagascar integrated into the African Union have historically been abortive. Furthermore, considering the various botched plans involved in the plot, the filmmakers undoubtedly intended the subtitle "Escape 2 Africa" to be somewhat ironic.
After the plane crash, the seat stuck to Gloria's butt has no shadows on the ground.
When Alex chooses to fight Teetsi the scene changes to Makunga making a comment to the lion on his left. Just as Makunga utters the words, "this ought to be good" the lion several rows in back and to the right of Makunga mouths the same words at the exact moment. This reveals how the animators use computer generated forms, animate them then simply place them in a scene with different skins.
In the very end of the movie, Alex glances at Marty's butt, which has a bite mark supposedly made by Alex in Madagascar (2005). However, the mark cannot be seen in any other scene that shows Marty's butt, such as the very first one when they are all shaking their butts with the music.