The first time that Charlotte sits down with Bob at the bar, Bob drinks almost all of his whiskey, but in the next shot it is nearly full again.
In the karaoke room, a shot of Charlotte speaking shows the room number as 601. In the next shot, Charlotte is sitting outside the room, smoking, and Bill comes out of a door now showing 602 instead of 601.
In the sushi restaurant, Charlotte's chopsticks repeatedly disappear and reappear between shots.
The pink flowers in Charlotte's room disappear when she is listening to the "soul searching" tape, but reappear later in the movie.
When arriving in Kyoto, there is a scene of what appears to be a 700 Series Shinkansen arriving at the station implying that Charlotte had been riding the 700 Series. However, when Charlotte is shown walking away from a train, it is a much older 100 Series Shinkansen.
A number of devices rely on the purported fact that Japanese mispronounces the letter 'R' as the letter 'L'. The opposite is true, Japanese mispronounces the letter 'L' as 'R' (Japanese doesn't contain L), while Chinese substitutes 'R' for 'L' (Chinese not containing an 'R' sound).
(at around 47 mins) When Bob and Charlotte get in the taxi, Bob is seen closing the rear door. In Japan, a taxi's doors are operated exclusively by the driver; passengers do not open nor close them.
The credits of the film incorrectly spell Jean-Benoît Dunckel as Jean-Benoît Dunckle. Jean-Benoît Dunckel is one half of the French band, Air.
In the shower, Bob turns the shower head counter-clockwise to tighten it. Like elsewhere in the world, knobs in Japan loosen counter-clockwise and tighten clockwise, suggesting the scene was flipped.
When Bob and Charlotte first see each other in the hotel elevator, a Japanese woman is seen wearing a kimono with the right flap covering the left. In Japan, this is only practiced with burial kimonos, indicating that the scene was most likely flipped.
On the day of departure, Bob goes to the telephone and leaves a message on Charlotte's answering machine, jokingly telling her that she stole his jacket. During the phone call, a woman comes out of a door behind him, revealing herself later as a big fan. The door she comes out leads to the Gentleman's toilet. In the wider shot when he goes to the phone, the indication can be read on the left side of the door.
At the end of the scene where Charlotte first sees Bob in the elevator, she does not actually exit the elevator. At the far right side of the screen, her purse is visible, revealing that she is just standing there.
When Suntory representatives hand Bob Harris their business cards during their first meeting at his hotel, several men use incorrect Japanese protocol. Japanese individuals exchange business cards(or handing anything to another person) using both hands with each thumb on the card top next to each other, not with one hand as shown. Also, they read over the card before putting it away and not just grab it and shove it in their pocket.
Charlotte criticizes Kelly for using "Evelyn Waugh" as a pseudonym, because Evelyn Waugh was a man. But Kelly pronounces "Evelyn" as a female name (with a short "E"), and so might just be using a gender flip to make the pseudonym more difficult to figure out. (Then again, she is portrayed as a bit too ditzy to be this clever.)
Most or all of the game audio heard in the arcade is not the original audio from the games shown.
In the beginning of the movie, the music from the piano in the hotel's restaurant does not match what the pianist is actually playing.
When Charlotte sits in her hotel room just before the "Are you awake?" message slides under her door, the camera, operator, boom mic and sound recordist are all reflected in the window behind her.
When Bob is sitting on the Hotel's bedroom bed, dressed in a green robe and white sleepers looking sad, the cameraman and a woman can be seen in the window's reflection above his head.
When Bob is on the phone to Lydia after he gets back from karaoke, she tells him that the kids are eating breakfast and she needs to get them off to school. In reality, the earliest it could be is 11 am on the west coast (when not during daylight savings) if it's 4 a.m. in Japan. This is too late for breakfast if the children are heading to school.
Bob Harris is shown arriving in Tokyo by car. The background sound includes a public service announcement identifying Narita Airport (East of Tokyo) as port of entry. The sequence ends with his car pulling into the Park Hyatt hotel in West Shinjuku. In between, POV shots of Tokyo are shown from inside his car, but they are shots which would be seen by someone heading east out of the West Shinjuku Park Hyatt area, not entering from east into the area. Arrival at Park Hyatt from either of Tokyo's airports would be straight off of Shinjuku's highway interchange, not passing any of what is shown; i.e., leaving the West Shinjuku neighborhood, driving east away from Park Hyatt under the Yamanote train line into the bright lights lining Yasukuni Road where the Kabukicho red light district begins in East Shinjuku.
When Charlotte arrives at Shibuya Crossing, the camera shot from her point of view suggests she is crossing away from Shibuya Station, viewing the dinosaur on the jumbotron across the street. When she actually is seen crossing the street, however, she is walking towards the station.
In the opening scene when Bob Harris is arriving in his taxi, he is looking out the window at the bright lights of Shinjuku where his hotel is located. The one sign he notices however (the red bannered kanji with the blue circular lights) is a very distinctive sign located at Shibuya Crossing.
When Charlotte takes the subway, the orange "you are here" circle on the map indicates that she's at Shibuya station. When she gets to the platform, a sign says she's at Omotesando station, one stop away on the Ginza line.
At the beginning of the scene where Bob is in the bath tub in his hotel room a boom mic is visible over the tub, peeking out from behind the wall in the foreground.
When John (Giovanni Ribisi) first runs into Kelly (Anna Faris) in the lobby of the hotel he calls her Anna.
Near the beginning, when Bob takes a shower in his hotel room, he struggles comically because the shower head is so low. (Presumably everything in Japan is adjusted to the (again, supposed) shorter stature of the Japanese people. This also is demonstrated in the elevator scene, where Bob is a head taller than everyone else.) The problem is that the shower head easily could have been set a full foot higher on the rod; Bob chose to secure it too low (presumably for comic effect).
Early in the movie, during her distress call home, it appears that Charlotte mentions she "even tried ikebana" (the Japanese art of flower arrangement), but in fact only does so later, while wandering about in the hotel.
When Bob and Charlotte share a last drink in the New York Bar of the Park Hyatt, Charlotte wears white pajamas under her black coat, while Bob dresses with his green kimono and white slippers, as seen earlier in the fire alarm scene. The dress code of the bar "kindly request(s) that all guests refrain from wearing: Dress shorts; Beach sandals; Male sportswear such as training jerseys, tank tops or other sleeveless garments".