During Season 1's title sequence, the Pound Puppies logo is shown and zooms out. During the same sequence, Cooler, Whopper, Bright Eyes, Nose Marie, and Howler are introduced and they bowed. Whopper is then chased by Katrina and Brattina and inaudibly tells the others that he was chased by a monster. In his thought bubble, the monster is revealed as Catgut and Bright Eyes is scared and runs to Holly. As Katrina, Brattina, and Catgut inch closer, Cooler backs at them and frightens them away. Nose Marie is seen wheeling a bunch of flowers with the Pound Puppies Logo, which irises out to Howler, whose record-like derby irises out to all the Pound Puppies who sang the lyrics to the end of the Pound Puppies theme song.
During Season 2's title sequence, the camera zooms out to a different Pound Puppies logo, this time with Cooler in the logo and Katrina's dog pound in the background. In one scene, The Pound Puppies(With Howler absent) introduce themselves. The scene irises out to another scene where Cooler rescues three puppies and gives them to new owners. In another scene, Katrina, Brattina, and Catgut are looking at a wanted poster they made with the Pound Puppies(Now including Howler) picture in it. Catgut slices the wanted poster and the Pound Puppies come out and are chased by Katrina. Katrina catches them and puts the Pound Puppies in a kennel. The Pound Puppies fall through a trap door and slide down into their headquarters via Pupscalator with Holly waiting for them. At the end of the sequence, the camera then pans up to the same Pound Puppies logo.