The punks open the cemetery gates when the entered the graveyard. They were still open when Tina goes to see Freddy and they were open when Chuck and Casie were being chased by zombies to the warehouse, and they were still open when Trash comes out as a Zombie, but when Burt and Spider are driving through the graveyard they crash through the closed gates.
When Frank locks the door to the freezer containing the frozen zombie, it has a white door. Later, when the door is opened by Freddie, after which Burt bludgeons the zombie with a red mining pick, the door is completely different, dark tarnished metal instead of painted white.
Starting with the scene where Frank and Freddy wake up on the floor of the basement, the water and sweat stains on Frank's shirt change, getting inconsistently larger and smaller.
Clu Gulager's character name is spelled as 'Burt' in both the credits and on the 'eye test' poster in Frank and Freddy's office. However, the note on the door leading into the warehouse Office (which reads "No Cash in Safe After 5:00PM!") is signed by 'Bert'.
When Ernie and Tina are in the attic, they have a bit of wood braced over the door. Just before Freddy bursts through the door, the wood has gone.
Ernie claims that the process of rigor mortis begins in the brain. This is not true as rigor mortis only occurs in muscle tissue and the brain is not composed of muscle. Initial onset DOES begin in the head however specifically the muscles in the eyelids, jaw bone and neck.
When Frank shows Freddie the Tarman in the drum, the corpse is dead but if the Tarman is in the tank with the 245 trioxin gas then he would be alive inside the drum especially since it is later revealed that it is impossible to kill a reanimated body unless you reduce it to a pile of ash.
The ash from the mortuary incinerator is implied to mix with the rain that is falling to make a zombie creating brew. Even though the amount of rain is shown to be a heavy downpour (several inches accumulate in the cemetery in a short amount of time) there is no way that the rain/ash mix could penetrate through six feet of soil and a coffin in the short time portrayed. Even heavy rains will penetrate no more than a few inches of soil. It takes months if not years for water to penetrate to lower layers of soil. This is even more the case given the soil type that is around the Louisville Ky region where the movie is supposed to take place.
Ernie tells Burt that the embalming business is not an exact science and is basically passed down by word of mouth. When in actuality embalming was practiced by the ancient Egyptians for centuries and modern embalming was perfected in the early 1900's, and has changed very little since.
When Burt is on the phone talking to the police and hears the zombies overpowering them, he calls the number on the canister without first hanging up.
(at around 48 mins) When the skeleton rises out of the grave, you can see the wires that make his mouth move.
(at around 55 mins) When the Zombie calls for more paramedics, you can see where the white paint on his hand ends and his real skin is visible.
The skeleton that rises out of ground in the final before credits has a perfect pair of blue eyes in it's skull. The human eye soft tissue and liquid and would have rotted completely away if it had been the ground long enough to fully skeletonize.
A great number of the zombies appear to be recently deceased, meaning they were buried in modern caskets. There is no way the zombie acid rain mix would be able to penetrate through six feet of soil and then leak into both a concrete grave vault and a casket, both of which are designed to keep out water. And, if this did manage to happen, the zombies are shown to have regular strength and would have no way to break out of both a sealed buried casket and a grave vault since this would require near superhuman strength.
(at around 1 hr 2 mins) Immediately after Trash emerges from the mud, the movie fades to a clip of rain with smoke rising. The clip is looping in forward and reverse, as you can see the rain and smoke rising and falling several times, pausing briefly between each cycle.
(at around 1 hr 6 mins) Dozens of zombies attack the paramedics in order to eat their brains. But when the cops show up, the zombies have disappeared and brains are literally pouring out of one paramedic's head. Were they not that hungry?
Around 44:32, when Tina first discovers Tarman, there's no blood on his teeth the first time he says "Brains!" The second time Tarman says "Brains!" his teeth are suddenly covered in blood. This is because this is a reused scene of Tarman when he says "More brains!" right after he bites into and kills Suicide, and after Spider throws a 1-gallon paint can at Tarman.
Zombies without lips clearly pronounce B and P sounds - sounds that, of course, require lips to make.
This movie takes place in Louisville, KY in July where the average daily temperature is 90 degrees. Yet for some reason, Burt is wearing a jacket.
(at around 1h 12 mins) When Burt and Spider are in the cop car, and they screech on the brakes to avoid hitting a horde of Living Dead, the camera man can be seen in the back seat of the car, as well as the camera (look for a red light).
(at around 1 hr 8 mins) At the moment when Ernie slaps Spider in the embalming room look to the left of Ernie. You can see just the head and back of someone under the embalming table who is mostly hidden by the sheet covering the end of the table. This person (puppeteer?) is most probably there to operate the half woman skeleton on top of the embalming table.
When Burt decapitates the cadaver with the hacksaw, the camera cuts to a close up shot showing five hands holding the cadaver dummy.
(at around 1h 5 mins) When the first two police officers arrive on the scene of the two disabled ambulances, a man with black curly hair is visible in the backseat of the cruiser (seen through the rear-view mirror).
When Freddy is being shown around the warehouse at the beginning of the film, the shadow of the camera crew can be seen on the shelves.
Although the titles and characters mention the setting as Louisville, Kentucky, the skyline of downtown L.A., including the much-photographed dome of City Hall, is clearly visible as the crew cruise toward the warehouse early on in the film.
Though the setting is supposed to be Louisville, Kentucky, not a single person who speaks has a typical accent of the region.
Early in the film, an entire rack of skeletons (with perfect teeth) is shown in the main warehouse bay. When the zombie gas is released and animates all of the dead and preserved animals in the warehouse, the skeletons appear to be unaffected. (Later novels and comic books about the film tried to explain this by stating the gas affected the cadaver's nervous system, so perhaps the skeletons did not reanimate since they were completely clean of anything except bone.)
One thing that is never explained is WHY Burt would hold on to the Trioxin canisters for all those years. Aside from being Army property, he knew they contained hazardous materials.
When Frank is explaining how the original Night of the Living Dead is based on a true story, he states that the true story occurred in 1969. However Night of the Living Dead (1968) was released in 1968, a year before the incident happened. In the original script Frank said the event happened in 1966, but Dan O'Bannon changed the line because he felt it would be better if the character was unreliable.
When the zombie radios for more paramedics in the background you see a zombie eating and then someone laid back on their elbows just chilling.
(at around 46mins) When the two paramedics arrive on scene to examine Frank and Freddy, one asks the other about his stethoscope working because he could not find a blood pressure. The second paramedic has the same problem. However, neither are seen with the stethoscopes in their ears.
Frank is reading an order for two female skeletons with perfect teeth. He makes a big deal about how to fill the order but only has Freddy pack up one skeleton.
The type of security, house, and amenities that the Colonel is shown to have, essentially a guarded full service compound, is the kind of accommodation that the military would normally only give to a three or four star general. A regular Colonel, although they would live in on-base housing, would never be given this type of lodging, regardless of their posting or assignment.