At the start of the film, Jonathan Harker's journal narration establishes that it's May. Later, when Van Helsing and Holmwood ask the border official about the date Dracula's hearse would have crossed the frontier (within a week of the events that began the film), the date is given as December 1st.
When Dracula appears in the Holmwood's foyer in the middle of the night, there are two coats visible hanging on pegs in the background, one of which is Mina's green fur-trimmed coat which she wore earlier. When Van Helsing and Holmwood enter a short while later, after Dracula's visit, both coats are missing.
The position of the bite marks on Jonathan Harker's neck changes considerably from the scene in which he is bitten to the scene in which he inspects them with the mirror.
The scar burned into Mina's palm by the cross changes its appearance completely when it is seen for a second time.
During the final fight scene when Peter Cushing jumps on the table after pulling away the curtains to let the sunlight in, he knocks over a pile of books that appear to be glued together. However, they are really tied with a cord, frequently done to make a stack of books easier to carry.
It was established at the very beginning during Harker's narration that he was there to put an end to Dracula's reign of terror, therefore he knew what Dracula was; yet he was genuinely surprised shortly after his arrival at Dracula's castle when the vampire woman begged for his help to escape. However, he was playing dumb to ensure he wouldn't immediately antagonize Dracula.
In Dracula's crypt, the now-aged vampire woman can be seen still breathing, even after she has supposedly been destroyed.
Harker's bedroom in the castle is so cold that the supposedly-undead Dracula's breath is visibly condensing into mist.
Dracula falls to the floor before the sunlight hits him.
When Van Helsing repels Lucy with a crucifix, her reflection is visible in its surface. As a vampire she should cast no reflection.
About a quarter of an hour into the film, immediately following the scene where Dracula attacks Harker and then carries the vampire woman out from the library, there is an establishing shot of the outside of Dracula's castle. It looks motionless, but on closer inspection, a dark figure can be seen rushing past the bushes around the entrance to the castle. The most probable explanation is that it is, in fact, the top of Dracula's carriage, and the shot was originally filmed for the scene a few minutes later when the carriage rushes past Van Helsing on his first trip to the castle. However, the carriage is so indistinguishable in long-shot, the editors obviously abandoned it and used it here instead.
This film is set in 1885; however, when Dr. Van Helsing puts his fur coat on young Tania, he tells her she looks like a "teddy bear", a term that wasn't coined until 1902.
In the tavern where Van Helsing tries to get information from the scared landlord, a bottle of Gordon's Gin is sitting alone on the bar shelf; not only does this seem an unlikely choice of tipple for a Transylvanian village tavern, but the bottle is modern.
The vampire woman who attacks Harker is wearing 20th-century high-heeled shoes.
Van Helsing's wax cylinder phonograph (as designated by Edison) is an 1889 model. The story is set in 1885.
Arthur donates blood to Mina by transfusion. In 1885 transfusions were considered risky and dubious. Blood types were not identified and cataloged until 1901.
When Van Helsing and Mina's husband are pursuing Dracula in their carriage, they stop when they find the body of a murdered coachman: the audio is mismatched. The sound of the carriage and horses in motion continues even after their coach has come to a complete stop.
In the final scene, a bright shaft of sunlight was painted onto a transparent panel that was attached to the camera. When Dr. Van Helsing jumps off the dining table with the candle sticks, his right arm eclipses the shaft of light, briefly confusing depth perception.
The coffin Dracula uses in the undertaker's cellar has a large cross on the lid. Dracula could not touch that lid to get into the coffin.
When Harker is in the cellar to kill Dracula, he sees Dracula in his coffin and the girl in hers. Any sensible person would have killed Dracula first, then the girl, but not Harker. Obviously, it would have been a short film if he had, but the writer could have given the audience a reason for that. Instead, it just makes Harker look like a fool.
When Dr. Van Helsing examines Lucy, both she and Mina refer to him as merely Dr. Helsing.