- Holds a black belt in kung fu.
- Plays piano.
- When John appeared as a guest star on Boston Public (2000), his real-life father, R.F. Daley (a Broadway veteran), portrayed his TV father.
- Singer/keyboardist in the alternative rock band Dayplayer.
- John's mother, Nancy Daley, is an accomplished musician, singer, and teacher.
- Plays drums in a band.
- John's father is actor R.F. Daley, known for many productions on Broadway and in regional theater as well as appearances on television.
- Was in a relationship with writer Corinne Kingsbury for three years before they married in 2016. In 2017, they welcomed a son, Basil Daley.
- Auditioned several times for "Les Miserables" on Broadway - but was never tall enough to play Gavroche. By the time he was tall enough, he had lost interest.
- Was an honor student.
- #67 in VH1 100 Greatest Kid Stars (2012).
- Abandoned his long-time role as Dr. Lance Sweets in Bones (2005) by his directional debut in Vacation (2015), together his friend Jonathan Goldstein.
- Met his co-writer, Jonathan Goldstein, because Daley's girlfriend is a friend of Goldstein's wife.
- Played "Danny" in a school play of "Grease" for Nyack Middle School.
- Experienced his first "unemployment" due to his choice to decline to work on a 2006 pilot because the producers failed to meet his quote, thereby establishing his credibility as a bankable television personality.
- He laughs off screen in two episodes of Freaks and Geeks, ""Chokin' and Tokin' and "Noshing and Moshing.".
- Lived in Nyack, New York.
- Met writer Corinne Kingsbury in 2013. The couple married in 2016. In early 2017, they welcomed a son, Basil.
- #94 on VH1's 100 Greatest Teen Stars
- John and Samm Levine, his Freaks and Geeks co-star,have the same humor, so Samm would make John laugh at things his character said by saying the line in a way it would make John laugh, or just rolling his eyes at something.. This happens a few times throughout the show, and all the times this happens are in the show.
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