- Plays piano, trumpet, bass guitar, as well as the harp which she learned to play for The Big Bang Theory (2007).
- Her grandparents were European refugees from the Holocaust, immigrating from Poland and Czechoslovakia/Hungary. Bialik was raised in the Reform Jewish religion.
- For her very first screen kiss Mayim locked lips with co-star Johnny Galecki on Sex, Lies and Teenagers (1991) when both were just 14-years-old.
- Was good friends with the late Bill Bixby. As he went public with his illness, he directed her sitcom, Blossom (1990), and continued working up until his death on 21 November 1993.
- Her first name means "water" in Hebrew.
- Is an accomplished dancer. She and Blossom (1990) co-star, Jenna von Oÿ took lessons in between breaks in the filming season.
- Gave birth to her 1st child, at age 29, son Miles Roosevelt Bialik Stone, on October 10, 2005. Child's father is her now ex-husband, Michael Stone.
- Contrary to popular belief, did not do her own singing for the role of young "C.C. Bloom" in Beaches (1988). Although Mayim can sing, Bette Midler decided she wanted to feature a singer who sounded more like she herself did at age eleven, and another singer was substituted.
- According to a 2009 interview, her great great grandfather was the nephew of Haim Nahman Bialik, a Russian poet, who moved to Tel Aviv in the 1920s and who is today considered the national poet of Israel.
- Sustained an injury to her right hand in a car accident in Los Angeles on August 15, 2012 although later tweeted she would "keep all my fingers" after reports her thumb was almost severed to where she also nearly lost the use of her right index finger. Afterward, on five episodes or more of season six, her right hand was hidden in certain ways and camera shots to conceal the injury and at times she wore a glove to cover the hand while healing.
- Name pronounced "My-im, Bee-ah-lick".
- Never watched an episode of the last three seasons of Blossom (1990).
- Music director for the UCLA Jewish a capella group "Shir Bruin" (2001)
- Adopted a whale, a wolf, and a manatee.
- Gave birth to her 2nd child, at age 32, son Frederick Heschel Bialik Stone, on August 15, 2008. Child's father is her now ex-husband, Michael Stone.
- Played the recurring role of a lesbian on Curb Your Enthusiasm (2000) in what is deemed part of her "comeback" to acting after years of college studies and motherhood.
- She has an Erdös-Bacon-Sabbath number of 11, which is among the lowest on the planet. Her Erdös-Bacon number is 7 (also one of the lower numbers on the planet with 15 people with lower scores and tied with 6 others.).
- Separated from her husband of 9 years, Michael Stone. (November 2012)
- She is not related to Wall Street Journal online columnist Carl Bialik.
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