During the opening of the musical, Deadpool asks the bartender if he's going to "sew his mouth shut". This is a reference to Deadpool's unpopular and confusing portrayal in "X-Men Origins: Wolverine" (2009).
The line "But lately I'm thinking I might hump The Hulk, tell me what is that like Romanoff?" is in reference to Black Widow and The Hulk's Romance seen in The Avengers: Age of Ultron (2015).
The Deadpool Musical was filmed over the course of two production days, and one pickup day.
Deadpool fights with 17 people in the video. The female heroes fight with 11.