The villains are the Weaponers of Qward. In the comics, Qward was located in the Antimatter Universe.
When Uncle Sam gives Batman a "patriotic" boost, Batman's costume bears a slight resemblance to the costume worn by Captain America, particularly the white wings on the sides of his cowl. He also picks up a circular shield from one of the bad guys and throws it much like Captain America.
When Uncle Sam tells Plastic Man "I want YOU!", he imitates the famous Uncle Sam posters which were common in the United States during war time and encouraged young men to join the American military.
This show features many American Patriotic themes including Uncle Sam voiced by Peter Renaday. Renaday was known for providing the voice of Henry the Bear in the Country Bear Jamboree shows playing at Disney theme parks in California and Florida. The Disney parks were also known for having American Patriotism on display in shows like Hall of Presidents, America Sings and American Adventure.