Many of the recurring human characters, such as Scientist Alder, Chef Brockett, Handyman Negri, Nurse Farmer, Miss Emilie, Joey Hollingsworth, Digger Digorum and Mr. Anybody all carried over into Mister Rogers' Neighborhood (1968), though all but Negri and Brockett were phased out in the first few seasons.
Night of the Living Dead director and writer George A. Romero got his start working on set.
Daniel Striped Tiger, King Friday XIII, X the Owl, Henrietta Pussycat, Grandpere and Lady Elaine Fairchilde all originated on The Children's Corner, Fred Rogers' first series from the mid-1950s. They were brought over to this new series, which also introduced new puppet characters Edgar Cooke, Donkey Hodie and Cornflake S. Pecially.
Many story-lines, such as Corny's factory burning down, Lady Elaine thinking Corny wants to marry her, X applying for OCS, were all later remade as episodes of Mister Rogers' Neighborhood (1968).
Ernie Coombs, in addition to other roles, also served as Fred Rogers' understudy. When Rogers decided to return to the United States, Coombs stayed in Canada to accept an offer for his own series, Butternut Square (1964), which ultimately evolved into the classic Canadian children's' TV series, Mr. Dressup (1967).